LOCUS       sequence-81782-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ 
            indicator with Kd = 15 nM, in Dictyostelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura
            T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
  TITLE     Spontaneous network activity visualized by ultrasensitive Ca(2+) 
            indicators, yellow Cameleon-Nano.
  JOURNAL   Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 
            2010 Aug 8.
  PUBMED    20693999
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1488"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "729-32"
COMMENT     Sequence Label: Yellow Cameleon-Nano15/pBig
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..156
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     303..323
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(412..440)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcagatatcg atggtgatgg ccaagtaaac tatgaagagt ttgtacaaat gatgacagca
       61 aaggggggag gtggctccaa gaggcgctgg aagaaaaact tcattgccgt cagcgctgcc
      121 aaccggttca agaagatctc cagctccggg gcactggagc tgatggatgg aggtgttcaa
      181 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      241 aaccattacc tgtcctatca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      301 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
      361 aaaggtggca gcggtggcat ggttagtaaa ggagaagaac ttttcactgg agttgtccca
      421 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      481 gaaggtgatg caacatacgg aaaacttacc cttaaattaa tttgcactac tggaaaacta
      541 cctgttccat ggccaacact tgtcactact ttaggttatg gtcttcaatg ctttgcgaga
      601 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      661 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      721 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      781 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcaca
      841 gcagacaaac aaaagaatgg aatcaaagca aacttcaaaa
//
