LOCUS       sequence-81787-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=600nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_600/pRSETB
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(284..361)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 agcagccgga tcaagcttcg aattcttact gctcgttctt cagcactctc tccacgaagc
       61 tcttgatgta cttgcccatc tcgtcggggg cgtcctcctg gaggaagtgc aggcccttca
      121 ccttcacgaa ctcggtgttg gggaacttct tggcgccctc cacgatggcg ttgctgaaga
      181 agccggggtc accctcgatg aacagcttgg gcaggtcgtc gctggctctc aggtaggcgt
      241 tgtagtttct cacgatctgc accacgtcgg gcttgccgag ctccagtgcc ccggagctgg
      301 agatcttctt gaaccggttg gcagcgctga cggcaatgaa gtttttcttc cagcgcctct
      361 tccccccctt tgctgtcatc atttgtacaa actcttcata gtttacttgg ccatcaccat
      421 cgatatctgc ttcccttatc atttcatcaa cttcttcatc tgttaacttc tccccgaggt
      481 ttgtcatgac gtgacgtaac tgagcagcgc tgatgtagcc gttcccatcc ttgtcaaaaa
      541 cacggaatgc ttctcggatt tcctcttcgc tgtctgtgtc cttcattttt ctagccatca
      601 tagtaagaaa ttcaggaaag taaatcgttc cattgccatc agcatcgact tcattgatca
      661 tatcctgcaa ttctgcttcc gttgggtttt gtccaagcga cctcataacg gtgccaagtt
      721 cctttgtggt gatggtgccg tccccatcct tgtcgaataa tgagaaggct tctttgaact
      781 ctgcaatctg ctcttctgtc agttggtcat gcatccatgg gcccttcacc agggggatct
      841 ctctgggcca gctcagggtg ggtcttctca cctcgccctt ctc
//
