LOCUS       sequence-8179-ex        4230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ03
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     944..964
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1670..1692)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1792..1811
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1889..1907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1975..2079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2080..2148,2149..2940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2298..2317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3111..3699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3600..3619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3853..3870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3987..4008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4023..4028,4029..4046,4047..4053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4061..4077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4066..4088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4085..4101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4085..4101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      121 gggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
      361 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
      421 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      481 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
      541 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
      601 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      661 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      721 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
      781 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      841 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      901 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      961 taacagctgc tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt
     1021 cctaggaatt tattttgata agtctcgcgt attttatttg taataatntt ataataagat
     1081 caatttacat ttatactgat aaaattgaca caattacact ttttattata aaatatttta
     1141 aaatatttct atcaaaaggt ttatttttgt tttaaatata atggtttttt ttgtaaaaaa
     1201 tacttatttc gtttgataat ctttattcag agatttgaat aattttaaat aaagtagttt
     1261 agttttcata taaaagttaa tggtatatat aaatatttaa atagtaaaga ttgaatcagt
     1321 attcaaaatg acacctaaaa gttattctat ttctagctat aacaataata ataaagataa
     1381 ttatgataca gatttagaaa aaaaaataag gttaaattaa ttaattctat ttttaattaa
     1441 aatgatttaa gggagttaaa tgaaacagaa acagattggg tctctatata tttagtttat
     1501 gtaatgttat tttttggtgg aatacaaata acggtatatt ttacttcaat gtggccatac
     1561 ttatcaacaa taaatagtaa ggcaaatgta gatactttag ggtgggttgt agcactagtc
     1621 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1681 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1741 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     1801 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     1861 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     1921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3241 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3961 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4021 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4081 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
     4141 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
     4201 atggatacgc taacaacttg gaaatgaaat
//
