LOCUS       sequence-81806-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pMA122 - peel-1 negative selection
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(13..336)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    341..365
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ataaagttgt gagacttttt tcttggcggc acaataaagt ttcctctttt tgttgggtca
       61 ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt tcgggtttaa
      121 agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga atcacatttt
      181 gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt cataaaataa
      241 taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga aagaagagtg
      301 atagagaaga agggaatgct tgaaaggatt ttgcatttat cccactttgt acaagaaagc
      361 tgggtctcat ggattttcaa cacttggatc aatatgagca gttgagttga gacaattaaa
      421 tcgtacagca gcgcacaaag ttggtctcag gccagctgtt tgaactaatt ccatcaacgg
      481 atggatcgca ccacgagact cataataatt cagcagcaag gagaacagtg cagagttcaa
      541 tgataagagc atattctggc atgggtcaat gtaaaggaca tgggcgaaaa tctgtgcaag
      601 aaatatcaag ccaatcaaac ccttgcaaac aagaagcagg atagtttttg acgagacaga
      661 aaatcttcct tgcttaattt caaatgggct aaaaaaaatt aaccacaatg aagcaattga
      721 tccaataaga gcaataacat gcgcaaaccg atttacccat acattttcag gcagatcaag
      781 atacatgtag attttgaaaa tctttacaag agcaagtaaa aatccgacta tgatgttcca
      841 caaaaatatg aaaatcatcg aaaatttt
//
