LOCUS       sequence-81807-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pMA122 - peel-1 negative selection
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    527..551
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctatcaac tttgtataga aaagttgcta caggtcgact ctagaggatc accaaaaacg
       61 gaacgttgag ctggacggaa atagtggtaa agtgacatga ttatagtttg aagatttcta
      121 atttcacaat tagagcaaat gttgttcggt atttattttc aacggtattt atactatttt
      181 ccaccttttt ctagaacatt cgagctgctt gttgcaaaag gagggcgact cacattcggt
      241 acatggaaaa gtagtgtaca caataaagag acccagatac attttccgtc tgcgtctctt
      301 tgcacccacc gggagtattt tcaaacgaat gcatctagga ccttctagaa cattctgtaa
      361 ggctgcagaa tgcgggtata taaggaaagc gggctcagag gaagccaaca cgctttgttc
      421 tagtgcatct aaaaaacttc gaaaatcctc atcggatcct atcgattcgc ggccgctgta
      481 cacccgggtg ccctatagtg agtcgtattg gtaccggtag aaaaaatcaa gtttgtacaa
      541 aaaagcaggc ttcatgcgct ttgatttcca aaacttaaaa ttttcgatga ttttcatatt
      601 tttgtggaac atcatagtcg gatttttact tgctcttgta aagattttca aaatctacat
      661 gtatcttgat ctgcctgaaa atgtatgggt aaatcggttt gcgcatgtta ttgctcttat
      721 tggatcaatt gcttcattgt ggttaatttt ttttagccca tttgaaatta agcaaggaag
      781 attttctgtc tcgtcaaaaa ctatcctgct tcttgtttgc aagggtttga ttggcttgat
      841 atttcttgca cagattttcg cccatgtcct tt
//
