LOCUS       sequence-81808-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..35)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    76..579
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        616..720
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(939..958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
       61 atggtttctt agacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat
      121 ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta
      181 gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt
      241 taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat
      301 taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg
      361 tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc
      421 aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca
      481 aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat atatttatat
      541 taaaaaattt aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact
      601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      721 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      781 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      841 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      901 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      961 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgac
//
