LOCUS       sequence-81809-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        44..442
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     446..462
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     446..462
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctcatagct tcaaaatgtt
       61 tctactcctt ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca
      121 aaacacccaa gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt
      181 acccgtacta aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga
      241 aaaaggcaat aaaaattttt atcacgtttc tttttcttga aaattttttt tttgattttt
      301 ttctctttcg atgacctccc attgatattt aagttaataa acggtcttca atttctcaag
      361 tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga
      421 aagcatagca atctaatcta agttttctag aactagtgga tcccccggga aaaatggaca
      481 agaagtactc cattgggctc gatatcggca caaacagcgt cggttgggcc gtcattacgg
      541 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      601 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      661 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      721 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      781 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      841 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      901 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      961 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
     1021 aactc
//
