LOCUS       sequence-8181-ex        5401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ08
FEATURES             Location/Qualifiers
     source          1..5401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(366..377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1339..1367)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2115..2135
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2841..2863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2963..2982
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3060..3078)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3146..3250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3251..3319,3320..4111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3469..3488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4282..4870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4771..4790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5024..5041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5158..5179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5194..5199,5200..5217,5218..5224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5232..5248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5237..5259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5256..5272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5256..5272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgatt atcgtagtga gaggacgtgt agagataata aatttcccaa cttaatctat
       61 tataggaact tagttaaaaa tgattgtatt ggtcctcttt gtgaaagtgt ggaaaagagg
      121 tacctcgtag tttttttctt ctcaattctc atcatcaccc tgtgtaacat cacatctcat
      181 atagagaaat cgcgactttt attgcttagc atatactttg taagttgttt tcactgaggc
      241 tttgtccatc acacgctaca cattatcgct gttattttta ttatttatat ttattagtaa
      301 taatattaaa taaacacata ggaattttgt ctttgttggc ccttccagcc taaatagtta
      361 taaaatctac cttctattat acatacgcat atgttattta aaaagttgaa gtgatttctc
      421 ttctacagaa actacacatt tatcttctta ttttcttcca aacatatagc aataataatt
      481 taaaactttt tcttactcct cccagagttt gttaagttga tcatatttaa aattcttctg
      541 tcttctacaa caaataatat atttattata acaccaacta cctcgttcca agtattacgt
      601 aggagcatat acataccaca atgcttagga aatggggcgg ttatgatata acattatttc
      661 aatgtaacat tcttttttac cacatatatt ttctactgaa ttctgaaaac tcaatttcaa
      721 acctcctata tatatatatt gttaggcact tctaagatta taattcattt aactctctta
      781 aatttatcta ttttaaattt ttcctttttt gtattattta tactattata tcgaaacttt
      841 attattacat gtactattta acttttattt tcttatataa taataatact aaataataac
      901 tgataatcgt atctgtagag attattgaat tgtgattagt aatgtaaagt aaatctatat
      961 taagatatct caaagatgca ctcaagttat ttgaaaaatt tcttatacat agattaatac
     1021 ttatattaag aaactttaaa aaaaagtttt aaataaaaaa aaaaattcac aattgtacta
     1081 ttttatcttg tcgttcagtt tatcaccgta gtatttttat ggctagtaat attcatcatt
     1141 tttagataaa ctaacttgct atagaaaatg tgtgacgaag aggttgccgc tgcaggtcga
     1201 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     1261 aacatagaac attttcagga ggacccttgg agggtaccgg tagaaaaaat gagtaaagga
     1321 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1381 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1441 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
     1501 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
     1561 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     1621 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1681 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
     1741 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1801 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1861 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta
     1921 ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat
     1981 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     2041 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     2101 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     2161 gatgaactat acaaatagca ttcgtagaat tcctaggaat ttattttgat aagtctcgcg
     2221 tattttattt gtaataatnt tataataaga tcaatttaca tttatactga taaaattgac
     2281 acaattacac tttttattat aaaatatttt aaaatatttc tatcaaaagg tttatttttg
     2341 ttttaaatat aatggttttt tttgtaaaaa atacttattt cgtttgataa tctttattca
     2401 gagatttgaa taattttaaa taaagtagtt tagttttcat ataaaagtta atggtatata
     2461 taaatattta aatagtaaag attgaatcag tattcaaaat gacacctaaa agttattcta
     2521 tttctagcta taacaataat aataaagata attatgatac agatttagaa aaaaaaataa
     2581 ggttaaatta attaattcta tttttaatta aaatgattta agggagttaa atgaaacaga
     2641 aacagattgg gtctctatat atttagttta tgtaatgtta ttttttggtg gaatacaaat
     2701 aacggtatat tttacttcaa tgtggccata cttatcaaca ataaatagta aggcaaatgt
     2761 agatacttta gggtgggttg tagcactagt cggccgtacg ggccctttcg tctcgcgcgt
     2821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2881 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2941 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     3001 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     3061 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3961 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     4681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4921 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4981 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5041 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5101 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5161 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5221 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5281 cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt aaattttcag
     5341 agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa
     5401 t
//
