LOCUS       sequence-81810-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..280)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     246..264
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(296..316)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg ccgcaaatta aagccttcga gcgtcccaaa
       61 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa
      121 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa
      181 atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg
      241 ggagggcgtg aatgtaagcg tgacataact aattacatga ctcgagaaga gatcacacct
      301 tcctcttctt cttggggtca gccctgctgt ctccaccgag ctgagagagg tcgattcttg
      361 tttcatagag ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag
      421 aggtgtaccg ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca
      481 agttggtcag agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc
      541 tgtgcttatt gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt
      601 tggagaattc gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga
      661 acagctgctt ctgctcatta tcttcgggag accctttgag cttttcatag tggctggcca
      721 gatacaagaa attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc
      781 ccgcactagc gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa
      841 gcttaatgat gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg
      901 ggtttttttc gaagcttgat cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt
      961 tgagtttttt agacttccct ttctccactt tggccacaac cagtacactg taagcgactg
     1021 tagga
//
