LOCUS       sequence-81811-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..232
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             233..907
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta
       61 ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct ttttttgccg
      121 attaagaatt aattcggtcg aaaaaagaaa aggagagggc caagagggag ggcattggtg
      181 actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga gtatgtctgt
      241 tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga
      301 ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat gtgtgcccaa
      361 tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg taaaagcata
      421 taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc aacctaagga
      481 ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc atggagatga
      541 gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac tcgtatttcc
      601 aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt ttattccctt
      661 gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga tttctgactg
      721 ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg gactgacgcc
      781 agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg
      841 tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa
      901 gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt gcc
//
