LOCUS       sequence-81812-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt
       61 cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga
      121 tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg gcacgatatg
      181 atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct
      241 gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg
      301 gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct
      361 ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac
      421 gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt
      481 ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag
      541 actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc
      601 atcatggatc aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag
      661 gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc
      721 ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc
      781 gtaagttttc aagcgttctt caatcatctc cctatcttca aacaacgtaa gggtgaggac
      841 aatgtcctca agaatgtcct cgttctcctc attgtccagg aagtccttgt ctttaatgat
      901 tttcaggaga tcgtgatacg ttcccaggga tgcgttgaag cgatcctcca ctccgctgat
      961 ttcaacagag tcgaaacatt caatcttttt gaaatagtct tctttgagct g
//
