LOCUS       sequence-81816-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=150nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_150/pRSETB
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(285..362)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tagcagccgg atcaagcttc gaattcttac tgctcgttct tcagcactct ctccacgaag
       61 ctcttgatgt acttgcccat ctcgtcgggg gcgtcctcct ggaggaagtg caggcccttc
      121 accttcacga actcggtgtt ggggaacttc ttggcgccct ccacgatggc gttgctgaag
      181 aagccggggt caccctcgat gaacagcttg ggcaggtcgt cgctggctct caggtaggcg
      241 ttgtagtttc tcacgatctg caccacgtcg ggcttgccga gctccagtgc cccggagctg
      301 gagatcttct tgaaccggtt ggcagcgctg acggcaatga agtttttctt ccagcgcctc
      361 ttccccccct ttgctgtcat catttgtaca aactcttcat agtttacttg gccatcacca
      421 tcgatatctg cttcccttat catttcatca acttcttcat ctgttaactt ctccccgagg
      481 tttgtcatga cgtgacgtaa ttcagcagcg ctgatgtagc cgttcctatc cttgtcaaaa
      541 acacggaatg cttctcggat ttcctcttcg ctgtctgtgt ccttcatttt tctagccatc
      601 atagtaagaa attcaggaaa gtaaatcgtt ccattgccat cagcatcgac ttcattgatc
      661 atatcctgca attctgcttc cgttgggttt tgtccaagcg acctcataac ggtgccaagt
      721 tcctttgtgg tgatggtgcc gtccccatcc ttgtcgaata atgagaaggc ttctttgaac
      781 tctgcaatct gctcttctgt cagttggtca tgcatccatg ggcccttcac cagggggatc
      841 tctctgggcc agctcagggt gggtcttctc acctcgccct
//
