LOCUS       sequence-8182-ex        7537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ09
FEATURES             Location/Qualifiers
     source          1..7537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3475..3503)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4251..4271
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4977..4999)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5099..5118
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5196..5214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5282..5386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5387..5455,5456..6247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5605..5624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6418..7006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6907..6926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7160..7177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7294..7315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7330..7335,7336..7353,7354..7360)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7368..7384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7373..7395
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7392..7408
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7392..7408
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttcgca tgttggaatc tatgaggtta tttgaatcaa tttgtaactc tcgatggttt
       61 ataaatacat ctatgatact ttttcttaac aaaaaagatc ttcctgtaat tcatgattct
      121 atgacaaatg aattcataac caatttagta acagaatcta tatggattgg tcttacttgt
      181 agtgatacaa atacatgtaa ttggattgac aatagttctt ataattataa taattttcaa
      241 aatggattac cacaagtata tattggtgaa gcagttaaat tattaattca tcatgaacaa
      301 caaaatttta atggattatg gatttcatct tcatctaata atacaaatgc aacaacattg
      361 tgtagaaaac cacctaaagt tatatcacaa gaatgtcctg taaattatta ttacatttca
      421 aatgaaaata ataattgtta tagatatgtt gataatccaa aaacatttga tgatgcagca
      481 atagattgta aaaatgatgg cggtaattta gtttcaattc attcaaaaaa agaaaatact
      541 aacttattaa attttcttca aattatatca agaaaaaatt cagcatggat tggattacat
      601 tatgataata caaattcaaa atggttagat ggatcagagt atgattttaa caattataaa
      661 aaaggtaaca attttaataa ataatgatat aaattttata gactttccaa ataatctttt
      721 tggtaacggt gttgagattc ttctcgtaca agatgatgat ttatatgggt actgggaaaa
      781 tacttattta acaaataatc ttacatatat atgcaaaatg acaggtatat atttttttaa
      841 tttatataat ataaattata gaaaatgagg tttataaata taataatgta ataacaacaa
      901 taagtccaca aattacaaca ttatcaccat caaataaatg tccaaatagc ccaatgtatg
      961 aaaataatgg ttatgtgagt taataaaaat ttttttatta atttcatttt tagataaatt
     1021 ctcctggtta tccatcattt tatggaaata atatagattg ttattactat ttaattgtac
     1081 ctaaagaatt ttatgttcaa tttaagatta ttgattttaa tttaggtgta ggagattcat
     1141 tagatattta tgatggaaat gatttaaata atataataat gtcattaaca aattcagata
     1201 acactaattc atctggatta attttaacaa caaaaaataa taattttatg gtaatacatt
     1261 ttcattctaa tggaaatcat gtaataaaaa atagtggctt caatggatat tttacaacat
     1321 acgataaagt tttaataaca acatcacttc cattattaag tacaacaatt tatgtagaaa
     1381 caactggtac taatgaatgt ccatcaaaag agtttactaa taactcagaa attattacat
     1441 caccaggata tcctaatcca tttggaccaa atttgctttg tacatatctt attaaagcac
     1501 caattggaaa aattatagca ttacaatttg aaaaaattca tataactcaa gataaggtaa
     1561 gtttagaaat ttatgatggg aaagggatgt cattaaatat gttaaaacgg ataactaaaa
     1621 atgataatgg aaaaattgtt ttggagcttt caaaatctaa ttatatgaca ataatttttt
     1681 atacttccgg aacagcaggg attaatggtg atttttggta tctatattat cgttttattt
     1741 aaaaataaat aacataaacc aatattttat attaataaaa taatttgtaa taataataat
     1801 ataaaattac tggccaattt tctacattaa tacctttaat tttttccata tgttgctagg
     1861 taattaatga acattttgtt gaacatatta aatcatatat aataagagtg tctcattata
     1921 tatttttatt tttcaataaa ataattagac atatcattat atttatatga cattatattt
     1981 atttacgtat cattataaaa attatttttt tttaagtttt tctcccatat aattgttata
     2041 atttacaaaa ttctgaagaa aaaaaaatcc acattcacat taaaattaaa tatttaatta
     2101 acttttgtaa tattttttta taatattgtt taactttttc tacacacatg tatacataaa
     2161 ttaatttttt tattattaca ttttaatcat tgtatatatt taattattga gactttaaaa
     2221 gcatatattt taaaaaataa tatctttaaa tatatataac ggtaatttaa taaatttatc
     2281 ataataaaaa aaaatattaa tattaataaa aacaatattc actaaaatat tttatgtctt
     2341 tttatattca agttaaaaaa aaagttttaa aaatttattt aaaaagatat aattcaaatg
     2401 atataattta tctaattaat ttattcctac catatttaat tcttgttgta ctctaatata
     2461 ttaatattaa ttgtacaaag attaataatt ttgtagtttt atatataaaa ataatatatt
     2521 gtatttaaat aaaaaatgcc ctttataata aatttttctt tttacatata atttattcaa
     2581 aacatatttt ttcttaattt accttgtata atatattaat ataataaaaa attctaataa
     2641 aaagttgtat caataactaa atatttttaa ttattaatta ataaatatat ataataaatt
     2701 ttctagcaaa aaattatttt tctgattttt tatattttca tatgattttt aattgttata
     2761 attttttttt ttatttttta ataataaaaa aattatattt attataaaag ttaatgtaac
     2821 ctaggtaact ttaatatgat ctttttatca ctaatagtat gttaaataat gttaaaagtt
     2881 aattaatgat ttttggctaa ttttattgta attatatttt aattatggca tttataaaaa
     2941 gttattttag taatagatga cagttttcct ataataaaga taaatttata aaaatattta
     3001 aacgttatat gataatatta attatcattc aaatacttaa aactgttact tgcgtaattt
     3061 cctaactata agttgcttgg caactaaact ttttatatgt aatacaataa agttgtaatg
     3121 agtttattac ttataattaa aataatacaa aaaaattact tttaaatatg ttttaaaata
     3181 tttatatatt tttaaaaata actaaattat aaacaaatat ttttatttaa atttataaaa
     3241 gtagatttga tttttttttg attttagaat ttgttaaatt tcttattatt attataatat
     3301 tttattaata tgggagtttg tcaatcaaag tcgactctag aggatccccg ggattggcca
     3361 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
     3421 ccttggaggg taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc
     3481 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     3541 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     3601 ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     3661 tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca
     3721 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     3781 agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt
     3841 actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc
     3901 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     3961 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     4021 aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     4081 tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     4141 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     4201 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     4261 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa atagcattcg
     4321 tagaattcct aggaatttat tttgataagt ctcgcgtatt ttatttgtaa taatnttata
     4381 ataagatcaa tttacattta tactgataaa attgacacaa ttacactttt tattataaaa
     4441 tattttaaaa tatttctatc aaaaggttta tttttgtttt aaatataatg gttttttttg
     4501 taaaaaatac ttatttcgtt tgataatctt tattcagaga tttgaataat tttaaataaa
     4561 gtagtttagt tttcatataa aagttaatgg tatatataaa tatttaaata gtaaagattg
     4621 aatcagtatt caaaatgaca cctaaaagtt attctatttc tagctataac aataataata
     4681 aagataatta tgatacagat ttagaaaaaa aaataaggtt aaattaatta attctatttt
     4741 taattaaaat gatttaaggg agttaaatga aacagaaaca gattgggtct ctatatattt
     4801 agtttatgta atgttatttt ttggtggaat acaaataacg gtatatttta cttcaatgtg
     4861 gccatactta tcaacaataa atagtaaggc aaatgtagat actttagggt gggttgtagc
     4921 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4981 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5041 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5101 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5161 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     5221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6781 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7261 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7321 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7381 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
     7441 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
     7501 cacaacgatg gatacgctaa caacttggaa atgaaat
//
