LOCUS       sequence-81824-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=63nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_63/pRSETB
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(281..358)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 agccggatca agcttcgaat tcttactgct cgttcttcag cactctctcc acgaagctct
       61 tgatgtactt gcccatctcg tcgggggcgt cctcctggag gaagtgcagg cccttcacct
      121 tcacgaactc ggtgttgggg aacttcttgg cgccctccac gatggcgttg ctgaagaagc
      181 cggggtcacc ctcgatgaac agcttgggca ggtcgtcgct ggctctcagg taggcgttgt
      241 agtttctcac gatctgcacc acgtcgggct tgccgagctc cagtgccccg gagctggaga
      301 tcttcttgaa ccggttggca gcgctgacgg caatgaagtt tttcttccag cgcctcttgg
      361 agccacctcc cccctttgct gtcatcattt gtacaaactc ttcatagttt acttggccat
      421 caccatcgat atctgcttcc cttatcattt catcaacttc ttcatctgtt aacttctccc
      481 cgaggtttgt catgacgtga cgtaattgag cagcgctgat gtagccgttc ccatccttgt
      541 caaaaacacg gaatgcttct cggatttcct cttcgctgtc tgtgtccttc atttttctag
      601 ccatcatagt aagaaattca ggaaagtaaa tcgttccatt gccatcagca tcgacttcat
      661 tgatcatatc ctgcaattct gcttccgttg ggttttgtcc aagcgacctc ataacggtgc
      721 caagttcctt tgtggtgatg gtgccgtccc catccttgtc gaataatgag aaggcttctt
      781 tgaactctgc aatctgctct tctgtcagtt ggtcatgcat ccatgggccc ttcaccaggg
      841 ggatctctct gggccagctc agggtgggtc ttctcacctc
//
