LOCUS       sequence-81825-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Hygromycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-hygro
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(137..156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctttttct ggagactaaa taaaatcttt tattttatcg atgcgttgct ggcgtttttc
       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgtaac caccacaccc gccgcatcga tagatcccgg
      661 tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
      721 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
      781 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
      841 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
      901 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
      961 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     1021 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     1081 cgtcaggaca ttgttg
//
