LOCUS       sequence-81826-e        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Zeocin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-zeo (pBABE-bleo)
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..420)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(430..759)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      444..579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     744..764
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(838..854)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(870..892)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ccctttttct ggagactaaa taaaatcttt tattttatcg atcggtcagt cctgctcctc
       61 ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg
      121 ctgctcgccg atctcggtca tggccggccc ggaggcgtcc cggaagttcg tggacacgac
      181 ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc cacacccagg ccagggtgtt
      241 gtccggcacc acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac
      301 accggcgaag tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg tccagaactc
      361 gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg gcactggtca acttggccat
      421 ggtaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
      481 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
      541 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
      601 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
      661 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
      721 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacaggg tcgaccactg
      781 tgctggcgaa ttcctacgta ccaccacact gggatccggc cggcgcctag agaaggagtg
      841 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
      901 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
      961 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     1021 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gtta
//
