LOCUS       sequence-81828-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=11nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_11/pRSETB
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(285..362)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tagcagccgg atcaagcttc gaattcttac tgctcgttct tcagcactct ctccacgaag
       61 ctcttgatgt acttgcccat ctcgtcgggg gcgtcctcct ggaggaagtg caggcccttc
      121 accttcacga actcggtgtt ggggaacttc ttggcgccct ccacgatggc gttgctgaag
      181 aagccggggt caccctcgat gaacagcttg ggcaggtcgt cgctggctct caggtaggcg
      241 ttgtagtttc tcacgatctg caccacgtcg ggcttgccga gctccagtgc cccggagctg
      301 gagatcttct tgaaccggtt ggcagcgctg acggcaatga agtttttctt ccagcgcctc
      361 ttggagccac ctcccccctt tgctgtcatc atttgtacaa actcttcata gtttacttgg
      421 ccatcaccat cgatatctgc ttcccttatc atttcatcaa cttcttcatc tgttaacttc
      481 tccccgaggt ttgtcatgac gtgacgtaat tcagcagcgc tgatgtagcc gttcccatcc
      541 ttgtcaaaaa cacggaatgc ttctcggatt tcctcttcgc tgtctgtgtc cttcattttt
      601 ctagccatca tagtaagaaa ttcaggaaag taaatcgttc cattgccatc agcatcgact
      661 tcattgatca tatcctgcaa ttctgcttcc gttgggtttt gtccaagcga cctcataacg
      721 gtgccaagtt cctttgtggt gatggtgccg tccccatcct tgtcgaataa tgagaaggct
      781 tctttgaact ctgcaatctg ctcttctgtc agttggtcat gcatccatgg gcccttcacc
      841 agggggatct ctctgggcca gctca
//
