LOCUS       sequence-8183-ex        4246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ12
FEATURES             Location/Qualifiers
     source          1..4246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(164..192)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     940..960
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1686..1708)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1808..1827
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1905..1923)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1991..2095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2096..2164,2165..2956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2314..2333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3127..3715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3616..3635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3869..3886
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4003..4024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4039..4044,4045..4062,4063..4069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4077..4093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4082..4104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4101..4117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4101..4117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accggtagaa aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      181 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      241 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      301 atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac
      361 acttgtcact actttctgtt atggtgttca atgcttctcg agatacccag atcatatgaa
      421 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      481 tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc
      541 atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      601 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      661 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      721 agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt
      781 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      841 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      901 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      961 agctgctggg attacacatg gcatggatga actatacaaa tagcattcgt agaattacgc
     1021 gtcctaggcc tgaattccta ggaatttatt ttgataagtc tcgcgtattt tatttgtaat
     1081 aatnttataa taagatcaat ttacatttat actgataaaa ttgacacaat tacacttttt
     1141 attataaaat attttaaaat atttctatca aaaggtttat ttttgtttta aatataatgg
     1201 ttttttttgt aaaaaatact tatttcgttt gataatcttt attcagagat ttgaataatt
     1261 ttaaataaag tagtttagtt ttcatataaa agttaatggt atatataaat atttaaatag
     1321 taaagattga atcagtattc aaaatgacac ctaaaagtta ttctatttct agctataaca
     1381 ataataataa agataattat gatacagatt tagaaaaaaa aataaggtta aattaattaa
     1441 ttctattttt aattaaaatg atttaaggga gttaaatgaa acagaaacag attgggtctc
     1501 tatatattta gtttatgtaa tgttattttt tggtggaata caaataacgg tatattttac
     1561 ttcaatgtgg ccatacttat caacaataaa tagtaaggca aatgtagata ctttagggtg
     1621 ggttgtagca ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     1681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     1801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1861 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     1921 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1981 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2041 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2101 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2161 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2221 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2281 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2341 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2401 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2461 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2521 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2581 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2641 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2701 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2761 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2821 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2881 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2941 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3001 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3241 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3361 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3421 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3481 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     3541 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3601 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3661 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3721 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3781 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3841 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3901 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3961 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4021 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4081 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt
     4141 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
     4201 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
