LOCUS       sequence-81831-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=63nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_63/pcDNA3
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(321..398)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gaatagggcc ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat
       61 tcttactgct cgttcttcag cactctctcc acgaagctct tgatgtactt gcccatctcg
      121 tcgggggcgt cctcctggag gaagtgcagg cccttcacct tcacgaactc ggtgttgggg
      181 aacttcttgg cgccctccac gatggcgttg ctgaagaagc cggggtcacc ctcgatgaac
      241 agcttgggca ggtcgtcgct ggctctcagg taggcgttgt agtttctcac gatctgcacc
      301 acgtcgggct tgccgagctc cagtgccccg gagctggaga tcttcttgaa ccggttggca
      361 gcgctgacgg caatgaagtt tttcttccag cgcctcttgg agccacctcc cccctttgct
      421 gtcatcattt gtacaaactc ttcatagttt acttggccat caccatcgat atctgcttcc
      481 cttatcattt catcaacttc ttcatctgtt aacttctccc cgaggtttgt catgacgtga
      541 cgtaattgag cagcgctgat gtagccgttc ccatccttgt caaaaacacg gaatgcttct
      601 cggatttcct cttcgctgtc tgtgtccttc atttttctag ccatcatagt aagaaattca
      661 ggaaagtaaa tcgttccatt gccatcagca tcgacttcat tgatcatatc ctgcaattct
      721 gcttccgttg ggttttgtcc aagcgacctc ataacggtgc caagttcctt tgtggtgatg
      781 gtgccgtccc catccttgtc gaataatgag aaggcttctt tgaactctgc aatctgctct
      841 tctgtcagtt ggtcatgcat ccatggg
//
