LOCUS       sequence-81833-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=11nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_11/pcDNA3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(320..397)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 aatagggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
       61 cttactgctc gttcttcagc actctctcca cgaagctctt gatgtacttg cccatctcgt
      121 cgggggcgtc ctcctggagg aagtgcaggc ccttcacctt cacgaactcg gtgttgggga
      181 acttcttggc gccctccacg atggcgttgc tgaagaagcc ggggtcaccc tcgatgaaca
      241 gcttgggcag gtcgtcgctg gctctcaggt aggcgttgta gtttctcacg atctgcacca
      301 cgtcgggctt gccgagctcc agtgccccgg agctggagat cttcttgaac cggttggcag
      361 cgctgacggc aatgaagttt ttcttccagc gcctcttgga gccacctccc ccctttgctg
      421 tcatcatttg tacaaactct tcatagttta cttggccatc accatcgata tctgcttccc
      481 ttatcatttc atcaacttct tcatctgtta acttctcccc gaggtttgtc atgacgtgac
      541 gtaattcagc agcgctgatg tagccgttcc catccttgtc aaaaacacgg aatgcttctc
      601 ggatttcctc ttcgctgtct gtgtccttca tttttctagc catcatagta agaaattcag
      661 gaaagtaaat cgttccattg ccatcagcat cgacttcatt gatcatatcc tgcaattctg
      721 cttccgttgg gttttgtcca agcgacctca taacggtgcc aagttccttt gtggtgatgg
      781 tgccgtcccc atccttgtcg aataatgaga aggcttcttt gaactctgca atctgctctt
      841 ctgtcagttg gtcatgcatc catgggccct tcac
//
