LOCUS       sequence-8185-ex        4250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ14
FEATURES             Location/Qualifiers
     source          1..4250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     944..964
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1690..1712)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1812..1831
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1909..1927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1995..2099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2100..2168,2169..2960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2318..2337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3131..3719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3620..3639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3873..3890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4007..4028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4043..4048,4049..4066,4067..4073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4081..4097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4086..4108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4105..4121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4105..4121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      121 gggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
      361 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
      421 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      481 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
      541 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
      601 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      661 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      721 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
      781 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      841 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      901 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      961 taacagctgc tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt
     1021 acgcgtccta ggcctgaatt cctaggaatt tattttgata agtctcgcgt attttatttg
     1081 taataatntt ataataagat caatttacat ttatactgat aaaattgaca caattacact
     1141 ttttattata aaatatttta aaatatttct atcaaaaggt ttatttttgt tttaaatata
     1201 atggtttttt ttgtaaaaaa tacttatttc gtttgataat ctttattcag agatttgaat
     1261 aattttaaat aaagtagttt agttttcata taaaagttaa tggtatatat aaatatttaa
     1321 atagtaaaga ttgaatcagt attcaaaatg acacctaaaa gttattctat ttctagctat
     1381 aacaataata ataaagataa ttatgataca gatttagaaa aaaaaataag gttaaattaa
     1441 ttaattctat ttttaattaa aatgatttaa gggagttaaa tgaaacagaa acagattggg
     1501 tctctatata tttagtttat gtaatgttat tttttggtgg aatacaaata acggtatatt
     1561 ttacttcaat gtggccatac ttatcaacaa taaatagtaa ggcaaatgta gatactttag
     1621 ggtgggttgt agcactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     1681 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1741 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1861 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     1921 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1981 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2041 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2101 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2161 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2221 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2281 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2341 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2401 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2461 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2521 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2581 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2641 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2701 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2761 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2821 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2881 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2941 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3241 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     3541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3841 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3901 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3961 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4021 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4081 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     4141 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     4201 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
