LOCUS       sequence-81851-e        6239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid, pLXSN16E7, is used to produce amphotropic recombinant 
            retrovirus providing efficient gene transfers and expression of the 
            HPV16 E7 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Halbert CL, Demers GW, Galloway DA
  TITLE     The E7 gene of human papillomavirus type 16 is sufficient for 
            immortalization of human epithelial cells.
  JOURNAL   J Virol. 1991 Jan;65(1):473-8.
  PUBMED    1845902
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "1991-01"; volume: "65(1)"; pages: "473-8"
COMMENT     Sequence Label: pLXSN16E7
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             588..763
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    826..1025
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1226..1642
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1572..1594
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1610..1626
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2037..2057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2042..2371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2222..2357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2431..3225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2485..2504)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3095..3114
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             3285..3878
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3981..4003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4089..4229
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4144..4163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4244..4261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4415..5003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4495..4514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5174..5965,5966..6034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5797..5816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6035..6139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6207..6225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
      181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
      241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
      301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
      361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
      421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
      481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
      661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
      721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
      781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1621 cctcactcct tctctaggcg ccggaattcg agctcggtac ccggggatcc tctagagtcc
     1681 tgtaatcatg catggagata cacctacatt gcatgaatat atgttagatt tgcaaccaga
     1741 gacaactgat ctctactgtt atgagcaatt aaatgacagc tcagaggagg aggatgaaat
     1801 agatggtcca gctggacaag cagaaccgga cagagcccat tacaatattg taaccttttg
     1861 ttgcaagtgt gactctacgc ttcggttgtg cgtacaaagc acacacgtag acattcgtac
     1921 tttggaagac ctgttaatgg gcacactagg aattgtgtgc cccatctgtt ctcagaaacc
     1981 ataatctacc atggctgatc ctgcaggcat gcaagctaac tcgaggatcc ggctgtggaa
     2041 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2101 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     2341 aggctttttt ggaggcctag gcttttgcaa aaagcttggg ctgcaggtcg aggcggatct
     2401 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     2461 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     2521 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     2581 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     2641 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     2701 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     2761 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     2821 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     2881 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     2941 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     3001 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     3061 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     3121 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     3181 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     3241 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     3301 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     3361 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     3421 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     3481 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     3541 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     3601 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     3661 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     3721 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     3781 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     3841 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     3901 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt
     3961 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4021 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4081 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     4141 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4201 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     4261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5461 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     5521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5761 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     5821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
//
