LOCUS       sequence-81852-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Sirius, a pH-insensitive and photostable 
            ultramarine fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa
            K, Nagai T
  TITLE     An ultramarine fluorescent protein with increased photostability and
            pH insensitivity.
  JOURNAL   Nat Methods. 2009 May;6(5):351-3. doi: 10.1038/nmeth.1317. Epub 2009
            Apr 6.
  PUBMED    19349978
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1317"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "351-3"
COMMENT     Sequence Label: Sirius/pcDNA3
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..63)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(45..63)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(108..311)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(137..157)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        312..691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     863..882
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccttgctccc atcggatccg agctcggtac caagcttggg tctccctata gtgagtcgta
       61 ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata
      121 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      181 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      241 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      301 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      361 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      421 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      481 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      541 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      601 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
      661 tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga
      721 agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc
      781 cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg
      841 agcagatact ggcttaacta tgcggcatca gagcagattg tact
//
