LOCUS       sequence-81854-e        6442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid, pLXSN6bE6, is used to produce amphotropic recombinant 
            retrovirus providing efficient gene transfers and expression of the 
            HPV6b E6 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Halbert CL, Demers GW, Galloway DA
  TITLE     The E7 gene of human papillomavirus type 16 is sufficient for 
            immortalization of human epithelial cells.
  JOURNAL   J Virol. 1991 Jan;65(1):473-8.
  PUBMED    1845902
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "1991-01"; volume: "65(1)"; pages: "473-8"
COMMENT     Sequence Label: pLXSN6bE6
FEATURES             Location/Qualifiers
     source          1..6442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             588..763
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    826..1025
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1226..1642
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1572..1594
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1610..1626
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2240..2260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2245..2574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2425..2560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2634..3428
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2688..2707)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3298..3317
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             3488..4081
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4184..4206)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4292..4432
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4347..4366
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4447..4464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4618..5206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4698..4717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5377..6168,6169..6237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6000..6019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6238..6342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6410..6428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
      181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
      241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
      301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
      361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
      421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
      481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
      661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
      721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
      781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1621 cctcactcct tctctaggcg ccggaattcg agcaaccgaa aacggttgta tataaaccag
     1681 ccctaaaatt tagcaaacga ggcattatgg aaagtgcaaa tgcctccacg tctgcaacga
     1741 ccatagacca gttgtgcaag acgtttaatc tatctatgca tacgttgcaa attaattgtg
     1801 tgttttgcaa gaatgcactg accacagcag agatttattc atatgcatat aaacacctaa
     1861 aggtcctgtt tcgaggcggc tatccatatg cagcctgcgc gtgctgccta gaatttcatg
     1921 gaaaaataaa ccaatataga cactttgatt atgctggata tgcaacaaca gttgaagaag
     1981 aaactaaaca agacatctta gacgtgctaa ttcggtgcta cctgtgtcac aaaccgctgt
     2041 gtgaagtaga aaaggtaaaa catatactaa ccaaggcgcg gttcataaag ctaaattgta
     2101 cgtggaaggg tcgctgccta cactgctgga caacatgcat ggaagacatg ttaccctaaa
     2161 ggatattgta ttagacctgc aacctccaga ccctgtaggg ttacattgct atgagcaatt
     2221 agtaggggga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2281 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2341 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2401 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     2521 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     2581 gggctgcagg tcgaggcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     2641 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2701 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2761 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     2821 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2881 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     2941 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3001 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3061 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3121 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3181 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3241 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     3301 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     3361 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3421 tcttctgagc gggactctgg ggttcgataa aataaaagat tttatttagt ctccagaaaa
     3481 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3541 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3601 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3661 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3721 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3781 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3841 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3901 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3961 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     4021 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     4081 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
     4141 ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4261 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     4321 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4381 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     4441 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5041 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     5701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     6001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6361 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     6421 cacgaggccc tttcgtcttc aa
//
