LOCUS       sequence-81859-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-inactivating lentiviral plasmid for co-expression of your gene 
            of interest and dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     pHIV-dTomato
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHIV-dTomato
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(101..675,676..678,679..687)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(268..285)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     495..514
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gcggccgctg aactgaattc
       61 atcgacgtta actattctag agtacccggg ctaggatccg cccctctccc tccccccccc
      121 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
      181 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
      241 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
      301 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
      361 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
      421 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      481 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      541 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
      601 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      661 aaacacgatg ataatatggc cacaaccatg tcagtgagca agggcgagga ggtcatcaaa
      721 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
      781 gagggcgagg gcgagggccg cccctacgag ggcacccag
//
