LOCUS       sequence-8186-ex        4244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ15
FEATURES             Location/Qualifiers
     source          1..4244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(162..190)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     938..958
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1684..1706)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1806..1825
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1903..1921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1989..2093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2094..2162,2163..2954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2312..2331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3125..3713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3614..3633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3867..3884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4001..4022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4037..4042,4043..4060,4061..4067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4075..4091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4080..4102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4099..4115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4099..4115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagagg atccccggga ttggccaaag gacccaaagg
       61 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggagggtac
      121 cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      181 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      241 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      301 gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagccaacac
      361 ttgtcactac tttctgttat ggtgttcaat gcttctcgag atacccagat catatgaaac
      421 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      481 tcaaagatga cgggaactac aagacacgta agtttaaaca gttcggtact aactaaccat
      541 acatatttaa attttcaggt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      661 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      721 ttgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagaacttca
      781 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      841 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      901 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      961 ctgctgggat tacacatggc atggatgaac tatacaaata gcattcgtag aattacgcgt
     1021 cctaggcctg aattcctagg aatttatttt gataagtctc gcgtatttta tttgtaataa
     1081 tnttataata agatcaattt acatttatac tgataaaatt gacacaatta cactttttat
     1141 tataaaatat tttaaaatat ttctatcaaa aggtttattt ttgttttaaa tataatggtt
     1201 ttttttgtaa aaaatactta tttcgtttga taatctttat tcagagattt gaataatttt
     1261 aaataaagta gtttagtttt catataaaag ttaatggtat atataaatat ttaaatagta
     1321 aagattgaat cagtattcaa aatgacacct aaaagttatt ctatttctag ctataacaat
     1381 aataataaag ataattatga tacagattta gaaaaaaaaa taaggttaaa ttaattaatt
     1441 ctatttttaa ttaaaatgat ttaagggagt taaatgaaac agaaacagat tgggtctcta
     1501 tatatttagt ttatgtaatg ttattttttg gtggaataca aataacggta tattttactt
     1561 caatgtggcc atacttatca acaataaata gtaaggcaaa tgtagatact ttagggtggg
     1621 ttgtagcact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     1681 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     1741 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     1801 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     1861 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     1921 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2101 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2161 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2221 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2281 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2341 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2401 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2461 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2521 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2581 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2641 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2701 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2761 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2821 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2881 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2941 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3241 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3481 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3961 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4021 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4081 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt
     4141 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
     4201 aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
