LOCUS       sequence-81861-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM
  TITLE     Development of a self-inactivating lentivirus vector.
  JOURNAL   J Virol. 1998 Oct . 72(10):8150-7.
  PUBMED    9733856
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Oct . 72(10):8150-7."
COMMENT     Sequence Label: pLS-CG
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(480..524)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    709..942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
       61 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
      121 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
      181 actaatgacc ccgtaattga ttactattaa tcgaattcct gcagcccggg ggatccgttc
      241 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
      301 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
      361 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
      421 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
      481 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
      541 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
      601 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
      661 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
      721 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
      781 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
      841 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
      901 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
      961 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     1021 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt
     1081 ctgaagatca gcggccgctt gctgtgcgg
//
