LOCUS       sequence-81864-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             555..620
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 tccttctcta ggcgccggaa ttcgccagga tggaacacag cgggattctg gctagtctga
       61 tactgattgc tgttctcccc caagggagcc ccttcaagat acaagtgacc gaatatgagg
      121 acaaagtatt tgtgacctgc aataccagcg tcatgcatct agatggaacg gtggaaggat
      181 ggtttgcaaa gaataaaaca ctcaacttgg gcaaaggcgt tctggaccca cgagggatat
      241 atctgtgtaa tgggacagag cagctggcaa aggtggtgtc ttctgtgcaa gtccattacc
      301 gaatgtgcca gaactgtgtg gagctagact cgggcaccat ggctggtgtc atcttcattg
      361 acctcatcgc aactctgctc ctggctttgg gcatctactg ctttgcagga catgagaccg
      421 gaaggccttc tggggctgct gaggttcaag cactgctgaa gaatgagcag ctgtatcagc
      481 ctcttcgaga tcgtgaagat acccagtaca gccgtcttgg agggaactgg ccccggaaca
      541 agaaatccgg accggtgaaa cagactttga attttgacct tctcaagttg gcgggagacg
      601 tggagtccaa cccagggccc atggagcaga ggaagggtct ggctggcctc ttcctggtga
      661 tctctcttct tcaaggcact gtagcccaga caaataaagc aaagaatttg gtacaagtgg
      721 atggcagccg aggagacggt tctgtacttc tgacttgtgg cttgactgac aagactatca
      781 agtggctta
//
