LOCUS       sequence-81865-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            mAmetrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             559..624
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattcgcc aggatggaac acagcgggat tctggctagt
       61 ctgatactga ttgctgttct cccccaaggg agccccttca agatacaagt gaccgaatat
      121 gaggacaaag tatttgtgac ctgcaatacc agcgtcatgc atctagatgg aacggtggaa
      181 ggatggtttg caaagaataa aacactcaac ttgggcaaag gcgttctgga cccacgaggg
      241 atatatctgt gtaatgggac agagcagctg gcaaaggtgg tgtcttctgt gcaagtccat
      301 taccgaatgt gccagaactg tgtggagcta gactcgggca ccatggctgg tgtcatcttc
      361 attgacctca tcgcaactct gctcctggct ttgggcatct actgctttgc aggacatgag
      421 accggaaggc cttctggggc tgctgaggtt caagcactgc tgaagaatga gcagctgtat
      481 cagcctcttc gagatcgtga agatacccag tacagccgtc ttggagggaa ctggccccgg
      541 aacaagaaat ccggaccggt gaaacagact ttgaattttg accttctcaa gttggcggga
      601 gacgtggagt ccaacccagg gcccatggag cagaggaagg gtctggctgg cctcttcctg
      661 gtgatctctc ttcttcaagg cactgtagcc cagacaaata aagcaaagaa tttggtacaa
      721 gtggatggca gccgaggaga cggttctgta cttctgactt gtggcttgac tgacaagact
      781 atcaagtggc ttaaagacgg gagcataata agtcctctaa atgcaactaa aaacacatgg
      841 aatctgggca acaatgccaa agaccctcga ggcacgtatc a
//
