LOCUS       sequence-81866-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a bicistronic IRES-GFP containing retroviral vector, MCS is expanded
            from pMSCV-IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, 
            Vignali DA
  TITLE     Generation of T-cell receptor retrogenic mice.
  JOURNAL   Nat Protoc. 2006;1(1):406-17.
  PUBMED    17406263
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "406-17"
COMMENT     Sequence Label: pMSCV-IRES-GFP II (pMIG II)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    89..638
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(233..250)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     460..479
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(686..707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattcaga tcttacgtag ctagcggatc ccaattgctc
       61 gagcgggatc aattccgccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      121 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      181 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      241 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
      301 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
      361 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
      421 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
      481 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
      541 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
      601 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggtgagca agggcgagga
      661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca cc
//
