LOCUS       sequence-8187-ex        5196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ50
FEATURES             Location/Qualifiers
     source          1..5196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(366..377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2636..2658)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2758..2777
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2855..2873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2941..3045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3046..3114,3115..3906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3264..3283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4077..4665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4566..4585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4819..4836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4953..4974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4989..4994,4995..5012,5013..5019)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5027..5043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5032..5054
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5051..5067
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5051..5067
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgatt atcgtagtga gaggacgtgt agagataata aatttcccaa cttaatctat
       61 tataggaact tagttaaaaa tgattgtatt ggtcctcttt gtgaaagtgt ggaaaagagg
      121 tacctcgtag tttttttctt ctcaattctc atcatcaccc tgtgtaacat cacatctcat
      181 atagagaaat cgcgactttt attgcttagc atatactttg taagttgttt tcactgaggc
      241 tttgtccatc acacgctaca cattatcgct gttattttta ttatttatat ttattagtaa
      301 taatattaaa taaacacata ggaattttgt ctttgttggc ccttccagcc taaatagtta
      361 taaaatctac cttctattat acatacgcat atgttattta aaaagttgaa gtgatttctc
      421 ttctacagaa actacacatt tatcttctta ttttcttcca aacatatagc aataataatt
      481 taaaactttt tcttactcct cccagagttt gttaagttga tcatatttaa aattcttctg
      541 tcttctacaa caaataatat atttattata acaccaacta cctcgttcca agtattacgt
      601 aggagcatat acataccaca atgcttagga aatggggcgg ttatgatata acattatttc
      661 aatgtaacat tcttttttac cacatatatt ttctactgaa ttctgaaaac tcaatttcaa
      721 acctcctata tatatatatt gttaggcact tctaagatta taattcattt aactctctta
      781 aatttatcta ttttaaattt ttcctttttt gtattattta tactattata tcgaaacttt
      841 attattacat gtactattta acttttattt tcttatataa taataatact aaataataac
      901 tgataatcgt atctgtagag attattgaat tgtgattagt aatgtaaagt aaatctatat
      961 taagatatct caaagatgca ctcaagttat ttgaaaaatt tcttatacat agattaatac
     1021 ttatattaag aaactttaaa aaaaagtttt aaataaaaaa aaaaattcac aattgtacta
     1081 ttttatcttg tcgttcagtt tatcaccgta gtatttttat ggctagtaat attcatcatt
     1141 tttagataaa ctaacttgct atagaaaatg tgtgacgaag aggttgccgc tgcaggtcga
     1201 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     1261 aacatagaac attttcagga ggacccttgg agggtaccgg taaaaatggc atcatcagaa
     1321 gatgttatta aagaatttat gagattcaaa gttaaaatgg aaggttcagt taacggtcat
     1381 gaatttgaaa ttgaaggtga aggtgaaggt agaccatatg aaggtacaca aacagcaaaa
     1441 ttaaaagtta caaaaggtgg tccattacca tttgcatggg atattctttc accacaattt
     1501 caatatggta gtaaagcata tgttaaacat ccagcagata ttccagatta tcttaaactt
     1561 tcatttccag aaggttttaa atgggaaaga attatgaatt ttgaagatgg tggtgttgta
     1621 acagttactc aagattcaac attacaagat ggtgaattta tttataaagt taaattaaga
     1681 ggtaccaatt ttccatcaga tggtccagta atgcaaaaaa aaactatggg ttgggaagca
     1741 tcaacagaaa gaatgtatcc agaagatggt gcactaaaag gtgaaattaa aatgagatta
     1801 aaacttaagg atggtggtca ttatgatgca gaagttaaaa caacttataa agctaaaaaa
     1861 caagttcaat taccaggtgc ttataaaaca gatattaaat tagatattat ttcacataat
     1921 gaagattata ctattgttga acaatatgaa agagcagaag gtagacattc aacaggtgca
     1981 ggatctccta ggaatttatt ttgataagtc tcgcgtattt tatttgtaat aatnttataa
     2041 taagatcaat ttacatttat actgataaaa ttgacacaat tacacttttt attataaaat
     2101 attttaaaat atttctatca aaaggtttat ttttgtttta aatataatgg ttttttttgt
     2161 aaaaaatact tatttcgttt gataatcttt attcagagat ttgaataatt ttaaataaag
     2221 tagtttagtt ttcatataaa agttaatggt atatataaat atttaaatag taaagattga
     2281 atcagtattc aaaatgacac ctaaaagtta ttctatttct agctataaca ataataataa
     2341 agataattat gatacagatt tagaaaaaaa aataaggtta aattaattaa ttctattttt
     2401 aattaaaatg atttaaggga gttaaatgaa acagaaacag attgggtctc tatatattta
     2461 gtttatgtaa tgttattttt tggtggaata caaataacgg tatattttac ttcaatgtgg
     2521 ccatacttat caacaataaa tagtaaggca aatgtagata ctttagggtg ggttgtagca
     2581 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2641 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2701 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     2761 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2821 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     2881 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2941 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3001 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3061 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3121 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3181 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3241 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3301 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3361 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3421 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3481 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3541 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3601 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3661 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3721 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3781 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3841 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3901 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3961 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4021 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4201 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4441 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     4501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4741 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4801 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4921 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     4981 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5041 caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga
     5101 tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc
     5161 acaacgatgg atacgctaac aacttggaaa tgaaat
//
