LOCUS       sequence-81871-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of both TCRalpha and TCRbeta using a single 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, 
            Vignali DA
  TITLE     Generation of T-cell receptor retrogenic mice.
  JOURNAL   Nat Protoc. 2006;1(1):406-17.
  PUBMED    17406263
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "406-17"
COMMENT     Sequence Label: murine TCR OTI-2A.pMIG II
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             865..918
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattcaga tctaccatgg acaagatcct gacagcatcg
       61 tttttactcc taggccttca cctagctggg gtgaatggcc agcagcagga gaaacgtgac
      121 cagcagcagg tgagacaaag tccccaatct ctgacagtct gggaaggaga gaccgcaatt
      181 ctgaactgca gttatgagga cagcactttt aactacttcc catggtacca gcagttccct
      241 ggggaaggcc ctgcactcct gatatccata cgttcagtgt ccgataaaaa ggaagatgga
      301 cgattcacaa tcttcttcaa taaaagggag aaaaagctct ccttgcacat cacagactct
      361 cagcctggag actcagctac ctacttctgt gcagcaagtg acaactatca gttgatctgg
      421 ggctctggga ccaagctaat tataaagcca gacatccaga acccagaacc tgctgtgtac
      481 cagttaaaag atcctcggtc tcaggacagc accctctgcc tgttcaccga ctttgactcc
      541 caaatcaatg tgccgaaaac catggaatct ggaacgttca tcactgacaa aactgtgctg
      601 gacatgaaag ctatggattc caagagcaat ggggccattg cctggagcaa ccagacaagc
      661 ttcacctgcc aagatatctt caaagagacc aacgccacct accccagttc agacgttccc
      721 tgtgatgcca cgttgactga gaaaagcttt gaaacagata tgaacctaaa ctttcaaaac
      781 ctgtcagtta tgggactccg aatcctcctg ctgaaagtag ccggatttaa cctgctcatg
      841 acgctgaggc tgtggtcctc cggagagggc agaggaagtc tgctaacatg cggtgacgtc
      901 gaggagaatc ctggcccaat gtctaacact gtcctcgctg attctgcctg gggcatcacc
      961 ctgctatctt gggttactgt c
//
