LOCUS       sequence-81873-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-PI(4,5)P2 binding control for PH-PLCD1-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Varnai P, Balla T
  TITLE     Visualization of phosphoinositides that bind pleckstrin homology 
            domains: calcium- and agonist-induced dynamic changes and 
            relationship to myo-[3H]inositol-labeled phosphoinositide pools.
  JOURNAL   J Cell Biol. 1998 Oct 19;143(2):501-10.
  PUBMED    9786958
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1998-10-19"; volume: "143(2)"; pages: "501-10"
COMMENT     Sequence Label: PH-PLCD1(R40L)-GFP
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      587..596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(638..659)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(899..918)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattca
       61 cggcatggac tcgggccggg acttcctgac cctgcacggc ctacaggatg atgaggatct
      121 acaggcgctg ctgaagggca gccagctcct gaaggtgaag tcgacctcat ggaggagaga
      181 gctgttctac aagttgcagg aggactgcaa gaccatctgg caggagtccc gcaaggtcat
      241 gcggaccccg gagtcccagc tgttctccat cgaggacatt caggaggtgc gaatggggca
      301 ccgcacggag ggtctggaga agttcgcccg tgatgtgccc gaggaccgct gcttctccat
      361 tgtcttcaag gaccagcgca atacactaga cctcatcgcc ccatcgccag ctgatgccca
      421 gcactgggtg ctggggctgc acaagatcat ccaccactca ggctccatgg accagcgtca
      481 gaagctacag cactggattc actcctgctt gcgaaaagct gacaaaaaca aggacaacaa
      541 gatgagcttc aaggagctgc agaacttcct gaaggatcca ccggtcgcca ccatggtgag
      601 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      661 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      721 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      781 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      841 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      901 cgacggcaac tacaagaccc
//
