LOCUS       sequence-81874-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of both TCRalpha and TCRbeta using a single 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, 
            Vignali DA
  TITLE     Generation of T-cell receptor retrogenic mice.
  JOURNAL   Nat Protoc. 2006;1(1):406-17.
  PUBMED    17406263
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "406-17"
COMMENT     Sequence Label: murine TCR OTII-2A.pMIG II
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             865..921
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattcaga tctaccatgg acaagatcct gacagcatcg
       61 tttttactcc taggccttca cctagctggg gtgaatggcc agcagcagga gaaacgtgac
      121 caacagcagg tgagacaaag tccccaatct ctgacagtct gggaaggaga gaccgcaatt
      181 ctgaactgca gttatgagga cagcactttt aactacttcc catggtacca gcagttccct
      241 ggggaaggcc ctgcactcct gatatccata cgttcagtgt ccgataaaaa ggaagatgga
      301 cgattcacaa tcttcttcaa taaaagggag aaaaagctct ccttgcacat cacagactct
      361 cagcctggag actcagctac ctacttctgt gcagcaaggg gtaacagaat cttctttggc
      421 gatgggacgc agctggtggt gaagcccaac atccagaacc cagaacctgc tgtgtaccag
      481 ttaaaagatc ctcggtctca ggacagcacc ctctgcctgt tcaccgactt tgactcccaa
      541 atcaatgtgc cgaaaaccat ggaatctgga acgttcatca ctgacaaaac tgtgctggac
      601 atgaaagcta tggattccaa gagcaatggg gccattgcct ggagcaacca gacaagcttc
      661 acctgccaag atatcttcaa agagaccaac gccacctacc ccagttcaga cgttccctgt
      721 gatgccacgt tgactgagaa aagctttgaa acagatatga acctaaactt tcaaaacctg
      781 tcagttatgg gactccgaat cctcctgctg aaagtagccg gatttaacct gctcatgacg
      841 ctgaggctgt ggtccggctc cggagccacg aacttctctc tgttaaagca agcaggagac
      901 gtggaagaaa accccggtcc catgtctaac actgccttcc ctga
//
