LOCUS       sequence-81878-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Is a biosensor for PIP3/PI(3,4)P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Varnai P, Balla T
  TITLE     Visualization of phosphoinositides that bind pleckstrin homology 
            domains: calcium- and agonist-induced dynamic changes and 
            relationship to myo-[3H]inositol-labeled phosphoinositide pools.
  JOURNAL   J Cell Biol. 1998 Oct 19;143(2):501-10.
  PUBMED    9786958
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1998-10-19"; volume: "143(2)"; pages: "501-10"
COMMENT     Sequence Label: PH-Akt-GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      650..659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(701..722)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
       61 catcagaggc tgtggccagg ccagctgggc tcggggagcg ccagcctgag aggagcgcgt
      121 gagcgtcgcg ggagcctcgg gcaccatgag cgacgtggct attgtgaagg agggttggct
      181 gcacaaacga ggggagtaca tcaagacctg gcggccacgc tacttcctcc tcaagaatga
      241 tggcaccttc attggctaca aggagcggcc gcaggatgtg gaccaacgtg aggctcccct
      301 caacaacttc tctgtggcgc agtgccagct gatgaagacg gagcggcccc ggcccaacac
      361 cttcatcatc cgctgcctgc agtggaccac tgtcatcgaa cgcaccttcc atgtggagac
      421 tcctgaggag cgggaggagt ggacaaccgc catccagact gtggctgacg gcctcaagaa
      481 gcaggaggag gaggagatgg acttccggtc gggctcaccc agtgacaact caggggctga
      541 agagatggag gtgtccctgg ccaagcccaa gcaccgcgtg accatgaacg agtttgagta
      601 cctgaagctg ctgggcaagg gcactttcgg caaggtggat ccaccggtcg ccaccatggt
      661 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      721 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      781 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      841 gaccaccctg acctacggcg tgcagtgctt cagccgctac
//
