LOCUS       sequence-8188-ex        7413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ20
FEATURES             Location/Qualifiers
     source          1..7413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3351..3379)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4127..4147
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4853..4875)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4975..4994
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5072..5090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5158..5262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5263..5331,5332..6123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5481..5500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6294..6882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6783..6802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7036..7053
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7170..7191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7206..7211,7212..7229,7230..7236)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7244..7260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7249..7271
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7268..7284
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7268..7284
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttagct caacgtgacg gattggttcc taaataactc gtctattgaa attaatttca
       61 tttaatatgc ttgtttgtta tcgttgtgtt ctacaataaa gaagttgtta tagtaattta
      121 tatttatata tatatataga aatttattta aatttctttc attttcatca ttattattat
      181 taacaactat ttgtcctcta tctctgtaaa tctttaaaat ccacataacg gtaatgattc
      241 caataacaat tgaaagtcta tcaaaagtta cataattcat cattaacaat gttagagctg
      301 aaaatcggag gactgacaac aatataccca taaaaccaac acgtaatccg ttaaagtttc
      361 taacaatatc attatatcta acattatttc cttgattatt attatttcca ttatcaaaac
      421 ctccattatt tatattattt ggttgttgaa tatttgtttg aaaagatata ttcattgttg
      481 gaaatgaagg cattggtgtg gattctctta atcctaattc ttgacgtaaa gaattagcat
      541 agtaatgttg atattggatt aattgtgtca ggtacgtttg ataataaata tgatatgggt
      601 ttgaaggatc ataatttgta attattggtg taactaaacc aggtatattt gggattcctt
      661 ctgaggataa tgaaggctca ttagttgtag aattagcctc ctctgttaca ttagtatttt
      721 gtgtttgatc gtctctccta ttttgtgttt cattctcccc attttcgtca atatttctat
      781 tgtcattagt ttgtttttca attggaacaa ctaaatggat aatttttggc gtctccaaat
      841 ccaatgaatc atcattgctt tggtccgtta atgaggttga agtataattt tcacttaatt
      901 gtcgttgatg ggacattaat atatctctta atgtttgtga atcgtttaaa cattttccca
      961 gaaatattaa tttttgtttt gttgctttct aaatatttat atgtaaaaaa aatagtaata
     1021 acaaaacaaa aaagattaaa aagttaccgg atttgatgga caagtttctt ccaaatgttt
     1081 tttcatatca aaaacagtcc actccaaaga acatttaacc ttgtgatcat caaaagtttg
     1141 aaaagctgaa cgaatggtaa attcaacagt gtctatattt gatgaagttt cagactccat
     1201 tcttttaaat tacacaaaac aataatcttt ttgtattaga taaaaaaaat aataaaaaat
     1261 aatattatat atcgacaatt gtgacagaga caaaataata gttaaatgga attgattttt
     1321 atatctgcca gcaaggcgtt tctcttcgtg tatgtgtttc ctgcaatgaa taaatgtacg
     1381 ttgatgtcac tctatgtata tctctataaa gttattaaac atgttccgaa agacatgtaa
     1441 actttatcat tttaaatatt aatatttatt tcaaatttta gcttttttta cctattaaaa
     1501 tttattaata tttaataaat ttaaaaattt ggttgtacat tttttaataa aattattttt
     1561 caaagagttt tgttttaata taaaaactta aactacatac tataaaaaaa tattatatat
     1621 aatacacaga aatttataaa atattactct aaattagtaa taaaaactta aaatattagt
     1681 gtattgtata aaacattaat ttatatatgt ataatgtcta tataataatt aatttgttat
     1741 accacgtcat catactattc taatctaaca aattaaaaat ataggattga cgtatgattc
     1801 tttatttttt aatttacaca ataaatataa taatattttt tagatattat ttaactataa
     1861 ttacgtatta ctataatatt tttcataatt tatatagttt tataatttat aatgtatacg
     1921 ttaaaaagtg ttaaaaaatg tagatagatt ttttttaata tatattatta ttctaataat
     1981 cttacaaaaa aaaagtattt tttttatcaa aaaattgatt tatctatgtt aaaaaaaatt
     2041 agttgttatt actttaaaaa tagaattcca ctattactag gaagactttt aattgagtgt
     2101 catatcctgc gacgaaagat tgtcaaccct tttaaatata atatttatat agtaaataat
     2161 ttttttattc ttttaatgtt gatatttttt attaattcta taaaaaaatg tttatttcaa
     2221 ggataaatta atattgaaaa aattcgagga tgtcggattt tagaacattt tatgattcaa
     2281 tacctaaaat tacacgttac tggatgactg ggagtgtggt tatgccattg ttgggtcgta
     2341 taggtattct ttcaccctct tctatgtact taaattggca agatatttat gaaaattttc
     2401 atgtatgttt ttaaatcata ttcttaatat taataaaata ttttctttca gatttggaga
     2461 ccattaacag cattatttta ttatccatta acaccagcaa caggtttcaa ttggctttta
     2521 atgatgtatt ttatgtacaa ttattcgaag actgttgaga atgatttatt tacaagaaga
     2581 cctggagaat atttatttat gcttatattt ctttggctct ccagtgttgg gatatcgtta
     2641 gcatttggaa tatattttct tcttgaacca atggtattgg cagttctata ttactgggca
     2701 caaaataata aggataccat tgtatcattt tggtttggat cacgttttaa agctatgtac
     2761 ttgccatggg tattgttagg tttcaatatg attttaagag gtggtggtat taatgaattg
     2821 gctggcatca ttgttgggca tattttttat ttcgctacaa ttgagtatcc acttcaacat
     2881 ggtggaagac ctttattaac ttgtccacaa attttgtaag tattttttat ttgtttatta
     2941 ttattgtttt ttttttgtag aaaaacatac tttcctgatg aagttggggg atatcaatct
     3001 atgaatggtc caagagttac aggagatggt agatccggtt ctaataataa tcaatcacca
     3061 cgtacacatg catggggatc tggaagaact cttggtactg attaactatt tgtatatatt
     3121 ttttaaataa tttattataa gacttaaaat aaatttacaa gtcttatttt tttaattttt
     3181 atttaagaaa atatctggtt ttttttccaa atttatatta cttgttttac ttttattata
     3241 ttatttgtta tacatatatt atttaggttt atatattaca tttcgtcatg caaaacgaca
     3301 aaggagaacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa
     3361 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     3421 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     3481 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     3541 agccaacact tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc
     3601 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     3661 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta
     3721 actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg
     3781 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     3841 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     3901 gaatcaaagt tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
     3961 agaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     4021 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     4081 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     4141 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaatag cattcgtaga
     4201 attcctagga atttattttg ataagtctcg cgtattttat ttgtaataat nttataataa
     4261 gatcaattta catttatact gataaaattg acacaattac actttttatt ataaaatatt
     4321 ttaaaatatt tctatcaaaa ggtttatttt tgttttaaat ataatggttt tttttgtaaa
     4381 aaatacttat ttcgtttgat aatctttatt cagagatttg aataatttta aataaagtag
     4441 tttagttttc atataaaagt taatggtata tataaatatt taaatagtaa agattgaatc
     4501 agtattcaaa atgacaccta aaagttattc tatttctagc tataacaata ataataaaga
     4561 taattatgat acagatttag aaaaaaaaat aaggttaaat taattaattc tatttttaat
     4621 taaaatgatt taagggagtt aaatgaaaca gaaacagatt gggtctctat atatttagtt
     4681 tatgtaatgt tattttttgg tggaatacaa ataacggtat attttacttc aatgtggcca
     4741 tacttatcaa caataaatag taaggcaaat gtagatactt tagggtgggt tgtagcacta
     4801 gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4861 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4921 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4981 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     5041 ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt
     5101 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5161 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5281 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5341 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5401 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5461 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5521 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5581 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5641 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5701 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5761 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5821 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5881 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5941 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6001 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6061 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6121 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6181 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6421 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6661 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     6721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6901 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6961 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7021 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7081 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7141 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7201 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7261 tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct
     7321 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
     7381 acgatggata cgctaacaac ttggaaatga aat
//
