LOCUS       sequence-81886-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a bicistronic IRES-mCherry containing vector for mammalian 
            expression (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     Vignali lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pICherryNeo
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    111..660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(255..272)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     482..501
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 attacagctc ttaaggctag agtacttaat acgactcact ataggctagc ctcgagaatt
       61 cacgcgtggt acctctagag tcgagcggga tcaattccgc ccccccccta acgttactgg
      121 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
      181 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
      241 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
      301 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
      361 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
      421 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
      481 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
      541 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
      601 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
      661 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      721 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      781 gccgccccta cgagggcacc cagaccgcca agctgaa
//
