LOCUS       sequence-81889-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a bicistronic IRES-mCherry containing vector for mammalian 
            expression (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Vignali lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pICherryNeo
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             126..836
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(271..289)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     539..558
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(894..913)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
       61 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata
      121 ataccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      181 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      241 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      301 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      361 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      421 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      481 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      541 ccgtaatgca gaagaagacg atgggctggg aggcctcctc cgagcggatg taccccgagg
      601 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      661 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      721 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      781 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgg
      841 ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt
      901 ttggacaaac cacaactaga
//
