LOCUS       sequence-81891-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Biosensor for PtdIns4P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Hammond GR, Machner MP, Balla T
  TITLE     A novel probe for phosphatidylinositol 4-phosphate reveals multiple 
            pools beyond the Golgi.
  JOURNAL   J Cell Biol. 2014 Apr 7.
  PUBMED    24711504
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2014 Apr 7."
COMMENT     Sequence Label: mCherry-P4M-SidM
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(167..472)
                     /codon_start=1
                     /product="high-affinity phosphatidylinositol 4-phosphate 
                     binding module of SidM (DrrA) from Legionella pneumophila 
                     (Hammond et al., 2014)"
                     /label=P4M
                     /translation="TASTENFKNVKEKYQQMRGDALKTEILADFKDKLAEATDEQSLKQ
                     IVAELKSKDEYRILAKGQGLTTQLLGLKTSSVSSFEKMVEETRESIKSQERQTIKIK"
     primer_bind     complement(763..782)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
      121 tggatcccgg gcccgcggta ccgtcgagtc gacccgggaa ttcttatttt atcttaatgg
      181 tttgtctttc ttgagactta atactttctc ttgtttcctc aaccattttc tcaaatgaag
      241 acactgaact tgtctttaac cctaaaagct gggttgttaa cccttgcccc ttagccaaaa
      301 ttctatattc atctttactt ttcaattcag caacaatttg cttaaggctc tgttcatcgg
      361 tagcttcagc cagtttatcc ttgaaatcag ccaggatttc tgtttttaaa gcatcacctc
      421 gcatttgctg atatttttct ttaacatttt taaagttttc cgtgcttgcc gtggatctga
      481 gtccggactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      541 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
      601 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
      661 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
      721 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
      781 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      841 gcagggagga gtcctgggtc acggtcacca cgccgccgtc
//
