LOCUS       sequence-81892-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a bicistronic IRES-mAmetrine 1.1 containing vector for mammalian 
            expression (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     Vignali lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIAneo
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(123..125,126..128,129..842)
                     /codon_start=1
                     /product="photostable yellow fluorescent protein with a 
                     large Stokes shift, excitable with violet light (Ai et al.,
                     2008)"
                     /label=mAmetrine1.1
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVRGEGEGDATNGKLTL
                     KFICTSGELPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNAWDAYITADKQKNGIK
                     ANFKIEHNVKDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(900..919)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
       61 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
      121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aggttcagcg tgcgcggcga gggcgagggc gatgccacca
      241 acggcaagct gaccctgaag ttcatctgca cctccggcga gctgcccgtg ccctggccca
      301 ccctcgtgac caccctgtct tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 ccttcaagga cgacggcagc tacaggaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaacatg aacgcgtggg acgcgtatat cacggccgac aagcagaaga
      601 acggcatcaa agcgaacttc aagatcgagc acaacgtcaa ggacggcggc gtgcagctcg
      661 ccgacgcgta ccagcagaac acccccatcg gcgacggctc cgtgctgctg cctgacaacc
      721 actacctgag cttccagagc aagctgttca aagaccccaa cgagcagcgc gatcacatgg
      781 tcctgctgga gttcgttacc gccgccggga taactcccgg catggacgag ctgtacaagt
      841 aagcggccgc ttccctttag tgagggttaa tgcttcgagc agacatgata agatacattg
      901 atgagtttgg acaaaccaca actagaatgc agtg
//
