LOCUS       sequence-81913-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(641..697)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa aataacatat
       61 agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta gggggggggc
      121 ggaattgatc ccgctcgagc aattgttagc gaggggccag ggtctgcata tgcagggcat
      181 cataggtgtc cttggtggca gtgctgagac cctggtaaag gccatcgtgc cccttgcctc
      241 tccgcctctc gccttttgtg ccgatctcac tgtaggcttc tgccatcttg tctttctgca
      301 gtgcattgta tacgccttcc tgggggttcc tcctcctctg ctgtttgcct cccatctctg
      361 ggtcccgagc ccgcttcttc tccaagacgt catattcctc tcttcgccct agattgagct
      421 cattgtagag ctggttgggg tcctgcaggt tggcagcagt ctctgcactc ctgctgaatt
      481 ttgctctcag gtacagggct gtgatgatga ctccgtagat gaagaggatt ccatctagca
      541 agtagcagag tttggggtcc agcagaccaa agctctgtgc ctctgctcct gggaaccgca
      601 cgtggaggat gcaggcgaga acagacactt tccacttcat gggaccgggg ttttcttcca
      661 cgtctcctgc ttgctttaac agagagaagt tcgtggcgga tccgactgct ctctgattca
      721 ggccagaata caggtcccgc tggcctttgc ggatgggctc atagtctggg ttgggaacag
      781 gtggtggccg ctccttgttt tgccctctgg gcctgctacc a
//
