LOCUS       sequence-81915-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector has been discontinued as of 5/8/2014. See Comments below
            for information on the updated version of the lentiCRISPR vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, 
            Heckl D, Ebert BL, Root DE, Doench JG, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout screening in human cells.
  JOURNAL   Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. 
            Epub 2013 Dec 12.
  PUBMED    24336571
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1247005"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "84-7"
COMMENT     Sequence Label: lentiCRISPR
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..155
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
       61 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      121 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      181 gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa
      241 atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt
      301 taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa
      361 aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat
      421 aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc
      481 atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga
      541 cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt
      601 aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt
      661 taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc
      721 tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac
      781 catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc
      841 tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt
      901 taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa
      961 ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa
     1021 tgtgtgatct atatatttgt ttag
//
