LOCUS       sequence-81927-e        1344 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS12
FEATURES             Location/Qualifiers
     source          1..1344
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..120,121..165,166..171,172..441,442..459,460..894,
                     895..897,898..900,901..1344)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSWNKTGH
                     AVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLL
                     PDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFT
                     GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGV
                     QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQ
                     NPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI
                     SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..120,121..165,166..171,172..441,442..459,460..894,
                     895..897,898..900,901..1344)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSWNKTGH
                     AVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLL
                     PDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFT
                     GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGV
                     QCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQ
                     NPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI
                     SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     378..399
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(505..526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(766..785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcatgg
      121 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
      181 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
      241 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
      301 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
      361 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      421 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
      481 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      541 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
      601 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      661 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      721 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
      781 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      841 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacacgcgt
      901 gaccaactga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
      961 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     1021 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     1081 acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaaga cacagacagt
     1141 gaagaagaaa ttagagaagc gttccgtgtg tttgataagg atggcaatgg ctacatcagt
     1201 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     1261 gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     1321 gtacaaatga tgacagcgaa gtga
//
