LOCUS       sequence-81928-e        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS14
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..171,172..177,178..447,448..465,466..900,901..903,
                     904..906,907..1350)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     THAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..171,172..177,178..447,448..465,466..900,901..903,
                     904..906,907..1350)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     THAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     384..405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(511..532)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(772..791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
      121 cgtaagtgga ataagacaca cgcagtcaga gctataggtc ggctgagctc actcgagaac
      181 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
      241 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
      301 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa
      361 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      421 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
      481 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      541 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
      601 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      661 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      721 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
      781 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      841 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      901 acgcgtgacc aactgactga agagcagatc gcagaattta aagaggcttt ctccctattt
      961 gacaaggacg gggatgggac aataacaacc aaggagctgg ggacggtgat gcggtctctg
     1021 gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt
     1081 gacggcacaa tcgacttccc tgagttcctg acaatgatgg caagaaaaat gaaagacaca
     1141 gacagtgaag aagaaattag agaagcgttc cgtgtgtttg ataaggatgg caatggctac
     1201 atcagtgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt aacagatgaa
     1261 gaggttgatg aaatgatcag ggaagcagac atcgatgggg atggtcaggt aaactacgaa
     1321 gagtttgtac aaatgatgac agcgaagtga
//
