LOCUS       sequence-81929-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            mAmetrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(639..695)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
       61 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg gggggggcgg
      121 aattgatccc gctcgagcaa ttgttagcga ggggccaggg tctgcatatg cagggcatca
      181 taggtgtcct tggtggcagt gctgagaccc tggtaaaggc catcgtgccc cttgcctctc
      241 cgcctctcgc cttttgtgcc gatctcactg taggcttctg ccatcttgtc tttctgcagt
      301 gcattgtata cgccttcctg ggggttcctc ctcctctgct gtttgcctcc catctctggg
      361 tcccgagccc gcttcttctc caagacgtca tattcctctc ttcgccctag attgagctca
      421 ttgtagagct ggttggggtc ctgcaggttg gcagcagtct ctgcactcct gctgaatttt
      481 gctctcaggt acagggctgt gatgatgact ccgtagatga agaggattcc atctagcaag
      541 tagcagagtt tggggtccag cagaccaaag ctctgtgcct ctgctcctgg gaaccgcacg
      601 tggaggatgc aggcgagaac agacactttc cacttcatgg gaccggggtt ttcttccacg
      661 tctcctgctt gctttaacag agagaagttc gtggcggatc cgactgctct ctgattcagg
      721 cca
//
