LOCUS       sequence-81942-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Nolte H, Holper S, Housley MP, Islam S, Piller T, Konzer A, Stainier
            DY, Braun T, Kruger M
  TITLE     Dynamics of zebrafish fin regeneration using a pulsed SILAC 
            approach.
  JOURNAL   Proteomics. 2015 Feb;15(4):739-51. doi: 10.1002/pmic.201400316.
  PUBMED    25504979
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pmic"; 
            journalName: "Proteomics"; date: "2015-02"; volume: "15"; issue: 
            "4"; pages: "739-51"
COMMENT     Sequence Label: pGEM-and3 in situ probe
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(875..893)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(876..893)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(911..927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(911..927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(924..946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    935..951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(959..965,966..983,984..989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1004..1025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
       61 atggcggccg cgggaattcg attcgtcaga ggagatctgt ggatcccaac tggtatcgca
      121 aaaatcccga ctttcagtcc tactatcgtt actataatag tattgggcac acagaagggc
      181 tctatgagat cgataaaatt agaatgcttt accagcaaat gcgtcatctg gagctcacct
      241 atggccctga cgcatcgaaa taccagaata cccttggtct aaagccgacc cctccaccca
      301 caactcagcc tccaccccct actttaccac ctgtctccaa gatgaatgtt atatacctgt
      361 gcaattcaaa agacccccag tgcaagccac atattgtcta cctgcccgcc ggtggtgtgc
      421 ctgtactttg cgatccacgt taccatccaa actgtaaact aagtcctgcc cagaattctc
      481 cctcaccacc tgtaattgtc ccagaaccag tgacgactgt ccctcttcca ccaacacaaa
      541 aatccactcc accaccacct cctcctccta taatggttgt caaaggaatg gagtatgact
      601 gtgatcctta ctgggaccct gactgcctga tcgataatcc tccaaggcca gtaaaaatgg
      661 ttgaaccgtc atcagagctc ccagatgaga aggtgaaaaa ggaaattcca gtcgatccag
      721 tgccaaccta tgacccatat gacttcaatc aagacctgta tgaccccttt cgccatgctg
      781 ctccagctca ctaaaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag
      841 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg
      901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtc
//
