LOCUS       sequence-81943-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Nolte H, Holper S, Housley MP, Islam S, Piller T, Konzer A, Stainier
            DY, Braun T, Kruger M
  TITLE     Dynamics of zebrafish fin regeneration using a pulsed SILAC 
            approach.
  JOURNAL   Proteomics. 2015 Feb;15(4):739-51. doi: 10.1002/pmic.201400316.
  PUBMED    25504979
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pmic"; 
            journalName: "Proteomics"; date: "2015-02"; volume: "15"; issue: 
            "4"; pages: "739-51"
COMMENT     Sequence Label: pGEM-and4 in situ probe
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(882..899)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(917..933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(917..933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(930..952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    941..957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(965..971,972..989,990..995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1010..1031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
       61 atggcggccg cgggaattcg attcgcacag aactcttacc tgtccattca tcaccgacag
      121 atcatcctca gaagcagagc gcgcttaatg acgagtgtgt gtgtgtgtga gggtcagagg
      181 tcatgatgtc tgcggtgtgt atgctgtccg tcctgctgct gatctgcggt cagctggagg
      241 cgaaatccct gatggagatc ctcagcccgg atggagcgct cagtctggac tccagcaagg
      301 ctcatgagtt tctgtccagt tctcgaccca agcggagcct ggacccacgc tggcaccggc
      361 agagcccaga cttccaggcc tactacaaat actacagcag catcgggcac acagagggcc
      421 tgtatgaggt ggaccggatc cgcatgcagt accagcagat gcggcacctg gagcagctcc
      481 acggccccga tgcaccctac ttccagaacc ggctgggccg accggcgctt cctgcgcttc
      541 ccaaatgtga tcctgcaaca gacaagggct gcagagtgtc cccgccccca gccatagccc
      601 caccccccac cgcagtgaag ggccctgtgg ctccgccccc tgctgccgtg aagggccccg
      661 tggctccgcc cctcagtcag gcagatgtgg tgtatctgtg caatgctaaa gacccgctct
      721 gcaggccgca catcgtctac atgcccgcgg gggccgtccc ggtgctgtgc gacccacgct
      781 accatcccaa ctgcaagctg aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      841 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1021 aactcacatt aattgcgttg cgctcactgc ccgct
//
