LOCUS       sequence-81950-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             239..292
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aaactgcatt gagctaaaca taggcaccat atccggcttt atcttcgctg aggtcatcag
       61 catcttcttc cttgctcttg gtgtatatct cattgcggga caggatggag ttcgccagtc
      121 aagagcttca gacaagcaga ctctgttgca aaatgaacag ctgtaccagc ccctcaagga
      181 ccgggaatat gaccagtaca gccatctcca aggaaaccaa ctgaggaaga agagatctga
      241 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgcggtg
      301 gaacactttc tggggcatcc tgtgcctcag cctcctagct gttggcactt gccaggacga
      361 tgccgagaac attgaataca aagtctccat ctcaggaacc agtgtagagt tgacgtgccc
      421 tctagacagt gacgagaact taaaatggga aaaaaatggc caagagctgc ctcagaagca
      481 tgataagcac ctggtgctcc aggatttctc ggaagtcgag gacagtggct actacgtctg
      541 ctacacacca gcctcaaata aaaacacgta cttgtacctg aaagctcgag tgtgtgagta
      601 ctgtgtggag gtggacctga cagcagtagc cataatcatc attgttgaca tctgtatcac
      661 tctgggcttg ctgatggtca tttattactg gagcaagaat aggaaggcca aggccaagcc
      721 tgtgacccga ggaaccggtg ctggtagcag gcccagaggg caaaacaagg agcggccacc
      781 acctgttccc aacccagact atgagcccat ccgcaaaggc cagcggg
//
