LOCUS       sequence-81951-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of all four WT CD3 subunits, co-expressed with
            mAmetrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, 
            Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA
  TITLE     Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs 
            ensures effective negative selection and prevents autoimmunity.
  JOURNAL   Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 
            May 11.
  PUBMED    18469818
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1611"; 
            journalName: "Nat Immunol"; date: "2008-06"; volume: "9"; issue: 
            "6"; pages: "658-66"
COMMENT     Sequence Label: Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-z...
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..291
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aactgcattg agctaaacat aggcaccata tccggcttta tcttcgctga ggtcatcagc
       61 atcttcttcc ttgctcttgg tgtatatctc attgcgggac aggatggagt tcgccagtca
      121 agagcttcag acaagcagac tctgttgcaa aatgaacagc tgtaccagcc cctcaaggac
      181 cgggaatatg accagtacag ccatctccaa ggaaaccaac tgaggaagaa gagatctgag
      241 ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc aatgcggtgg
      301 aacactttct ggggcatcct gtgcctcagc ctcctagctg ttggcacttg ccaggacgat
      361 gccgagaaca ttgaatacaa agtctccatc tcaggaacca gtgtagagtt gacgtgccct
      421 ctagacagtg acgagaactt aaaatgggaa aaaaatggcc aagagctgcc tcagaagcat
      481 gataagcacc tggtgctcca ggatttctcg gaagtcgagg acagtggcta ctacgtctgc
      541 tacacaccag cctcaaataa aaacacgtac ttgtacctga aagctcgagt gtgtgagtac
      601 tgtgtggagg tggacctgac agcagtagcc ataatcatca ttgttgacat ctgtatcact
      661 ctgggcttgc tgatggtcat ttattactgg agcaagaata ggaaggccaa ggccaagcct
      721 gtgacccgag gaaccggtgc tggtagcagg cccagagggc aaaacaagga gcggccacca
      781 cctgttccc
//
