LOCUS       sequence-81968-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA 2 against rat Sema4D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: pSuper 44-2
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     40..56
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        76..290
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     211..230
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(363..379)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(364..380)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(408..426)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(447..463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(460..482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    471..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(495..501,502..519,520..525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc
       61 gggctgcagg aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt
      121 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac
      181 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa
      241 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac agatccccag
      301 cagacggagt gccttaattc aagagattaa ggcactccgt ctgctttttt ggaaaagctt
      361 atcgataccg tcgacctcga gggggggccc ggtaccagct tttgttccct ttagtgaggg
      421 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgc
//
